sir tubulin Search Results


sir tubulin  (Cytoskeleton Inc)
96
Cytoskeleton Inc sir tubulin
Sir Tubulin, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sir+tubulin/SiR-Tubulin+Kit/bio_rxiv__64898__2026__03__27__714708-235-5-6
Average 96 stars, based on 1 article reviews
sir tubulin - by Bioz Stars, 2026-09
96/100 stars
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sir tubulin  (Spirochrome)
96
Spirochrome sir tubulin
a) Mitotic progression in 293F cells. Cells were treated with Doxycycline (Dox) for 24 hours, or not treated (Ctrl), and imaged live with <t>SiR-Tubulin.</t> Representative still frames from LSFM videos in control and Dox-treated 293F cells are shown at different time points. Gaussian blur of 0.5 was applied to the images. Black arrows indicate the spindle pole just prior to metaphase onset. Red arrow indicates extra mitotic centriole which disappears at metaphase. Scale bar = 10 𝜇m. Note that in the total time (33 minutes) the Dox-treated cell does not progress beyond metaphase. b) Metaphase duration. Average metaphase time of cells which successfully completed mitosis. Quantification was made from FLSM timelapses. Mean ± s.d. from n = 31 ctrl, and 21 Dox-cells, n= 2 independent experiments. Students T-test shows significant difference. c, d ) Chromosome alignment during metaphase. Cells were treated with Doxycycline (Dox) for 24 hours, or not treated (Ctrl), fixed and stained with antibodies against tubulin (gray). DNA was visualized with DAPI (magenta). In ( c ) representative confocal images are shown. Arrowheads indicate misaligned chromosomes. Scale bar = 5 𝜇m. In ( d ) the percentage of spindles containing mis-aligned chromosomes at metaphase was quantified from. Shown is the mean ± s.d. from 33 control and 38 Dox-spindles, from 3 independent experiments. Students T-test shows significant difference. e, f ) Spindle length during metaphase. Cells were treated with Doxycycline (Dox) for 24 hours, or not treated (Ctrl), fixed and stained with antibodies against acetylated tubulin (gray). DNA was visualized with DAPI (magenta). In ( e ) representative confocal images are shown. Dotted lines indicate the pole-to-pole distance. Scale bar = 5 𝜇m. In ( f ) spindle length, or pole-to-pole distance was quantified from images such as depicted in ( e ). n= 28 control, and 33 Dox-induced cells from 3 independent experiments.
Sir Tubulin, supplied by Spirochrome, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sir+tubulin/SiR-tubulin/bio_rxiv__64898__2026__04__06__716648-285-51-52
Average 96 stars, based on 1 article reviews
sir tubulin - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier

Image Search Results


a) Mitotic progression in 293F cells. Cells were treated with Doxycycline (Dox) for 24 hours, or not treated (Ctrl), and imaged live with SiR-Tubulin. Representative still frames from LSFM videos in control and Dox-treated 293F cells are shown at different time points. Gaussian blur of 0.5 was applied to the images. Black arrows indicate the spindle pole just prior to metaphase onset. Red arrow indicates extra mitotic centriole which disappears at metaphase. Scale bar = 10 𝜇m. Note that in the total time (33 minutes) the Dox-treated cell does not progress beyond metaphase. b) Metaphase duration. Average metaphase time of cells which successfully completed mitosis. Quantification was made from FLSM timelapses. Mean ± s.d. from n = 31 ctrl, and 21 Dox-cells, n= 2 independent experiments. Students T-test shows significant difference. c, d ) Chromosome alignment during metaphase. Cells were treated with Doxycycline (Dox) for 24 hours, or not treated (Ctrl), fixed and stained with antibodies against tubulin (gray). DNA was visualized with DAPI (magenta). In ( c ) representative confocal images are shown. Arrowheads indicate misaligned chromosomes. Scale bar = 5 𝜇m. In ( d ) the percentage of spindles containing mis-aligned chromosomes at metaphase was quantified from. Shown is the mean ± s.d. from 33 control and 38 Dox-spindles, from 3 independent experiments. Students T-test shows significant difference. e, f ) Spindle length during metaphase. Cells were treated with Doxycycline (Dox) for 24 hours, or not treated (Ctrl), fixed and stained with antibodies against acetylated tubulin (gray). DNA was visualized with DAPI (magenta). In ( e ) representative confocal images are shown. Dotted lines indicate the pole-to-pole distance. Scale bar = 5 𝜇m. In ( f ) spindle length, or pole-to-pole distance was quantified from images such as depicted in ( e ). n= 28 control, and 33 Dox-induced cells from 3 independent experiments.

Journal: bioRxiv

Article Title: A role for tubulin in cellular quality control and proteostasis

doi: 10.64898/2026.04.06.716648

Figure Lengend Snippet: a) Mitotic progression in 293F cells. Cells were treated with Doxycycline (Dox) for 24 hours, or not treated (Ctrl), and imaged live with SiR-Tubulin. Representative still frames from LSFM videos in control and Dox-treated 293F cells are shown at different time points. Gaussian blur of 0.5 was applied to the images. Black arrows indicate the spindle pole just prior to metaphase onset. Red arrow indicates extra mitotic centriole which disappears at metaphase. Scale bar = 10 𝜇m. Note that in the total time (33 minutes) the Dox-treated cell does not progress beyond metaphase. b) Metaphase duration. Average metaphase time of cells which successfully completed mitosis. Quantification was made from FLSM timelapses. Mean ± s.d. from n = 31 ctrl, and 21 Dox-cells, n= 2 independent experiments. Students T-test shows significant difference. c, d ) Chromosome alignment during metaphase. Cells were treated with Doxycycline (Dox) for 24 hours, or not treated (Ctrl), fixed and stained with antibodies against tubulin (gray). DNA was visualized with DAPI (magenta). In ( c ) representative confocal images are shown. Arrowheads indicate misaligned chromosomes. Scale bar = 5 𝜇m. In ( d ) the percentage of spindles containing mis-aligned chromosomes at metaphase was quantified from. Shown is the mean ± s.d. from 33 control and 38 Dox-spindles, from 3 independent experiments. Students T-test shows significant difference. e, f ) Spindle length during metaphase. Cells were treated with Doxycycline (Dox) for 24 hours, or not treated (Ctrl), fixed and stained with antibodies against acetylated tubulin (gray). DNA was visualized with DAPI (magenta). In ( e ) representative confocal images are shown. Dotted lines indicate the pole-to-pole distance. Scale bar = 5 𝜇m. In ( f ) spindle length, or pole-to-pole distance was quantified from images such as depicted in ( e ). n= 28 control, and 33 Dox-induced cells from 3 independent experiments.

Article Snippet: We used the following non-standard reagents: Hygromycin B (50 mg/mL, Invitrogen, 10687-010), Puromycin (Sigma, P7255), Doxycycline hyclate (Sigma, D9891), DMEM (Gibco), Penicillin/Streptomycin (Gibco), Gibson Assembly Master Mix (NEB, E2611L), Protease Inhibitor Tablets EDTA-free (Roche Diagnostics, 11873580001), Ni-NTA Agarose beads (Qiagen, 30210), Ni-NTA Superflow Cartridge (1 mL) (Qiagen, 30721), Imidazole (Sigma, D1916), Sir-Tubulin (Spirochrome, sc002), Propidium Iodide (Invitrogen, P3566), Prolong Gold with DAPI (Life Technologies, P36931).

Techniques: Control, Staining